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Tags : Transplant   Lung preservation   Oxidative stress  more

Tags : Transplant   Lung preservation   Oxidative stress   Antioxidant enzyme   Lipid peroxidation   Rat.  less

Reference : 1. de Perrot M, Liu M, Waddell TK, Keshavjee S. Ischemia-reperfusioninduced lung injury. Am J Resp    more

Reference : 1. de Perrot M, Liu M, Waddell TK, Keshavjee S. Ischemia-reperfusioninduced lung injury. Am J Respir Crit Care Med. 2003;167(4):490- 511. 2. Al-Mehdi AB, Zhao G, Fisher AB. ATP-independent membrane depolarization with ischemia in the oxygen-ventilated isolated rat lung. Am J Respir Cell Mol Biol. 1998;18(5):653-661. 3. Institute of Laboratory Animal Research, Commission on Life Sciences, National Research Council. Guide for the care and use of laboratory animals. 6th ed.Washington: DC: National Academy Pres; 1996. 4. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-275. 5. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500. 6. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-169. 7. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-126. 8. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hyroxynonenal. In: Packer L, Glazer AN, eds. Methods in Enzymology. Oxygen Radicals in Biological Systems. 9th ed. New York, NY: Academic Press; 1990:407-421. 9. Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem. 1990;36(8 Pt 1):1440-1443. 10. Pryor WA, Houk KN, Foote CS, et al. Free radical biology and medicine: it's a gas, man! Am J Physiol Regul Integr Comp Physiol. 2006;291(3):R491-R511. 11. Rodriguez C, Mayo JC, Sainz RM, et al. Regulation of antioxidant enzymes: a significant role for melatonin. J Pineal Res. 2004;36(1):1-9. 12. Yang W, Block ER. Effect of hypoxia and reoxygenation on the formation and release of reactive oxygen species by porcine pulmonary artery endothelial cells. J Cell Physiol. 1995;164(2):414- 423. 13. Lee CY, Pastore JN, Tang G, Tsan MF. Cellular distribution of pulmonary Mn and CuZn superoxide dismutase: effect of hyperoxia and interleukin-1. J Histochem Cytochem. 1994;42(9):1201-1205. 14. Terada LS, Guidot DM, Leff JA, et al. Hypoxia injures endothelial cells by increasing endogenous xanthine oxidase activity. Proc Natl Acad Sci U S A. 1992;89(8):3362-3366. 15. Quinlan T, Spivack S, Mossman BT. Regulation of antioxidant enzymes in lung after oxidant injury. Environ Health Perspect. 1994;102(suppl 2):79-87. 16. Heffner JE, Repine JE. Pulmonary strategies of antioxidant defense. Am Rev Respir Dis. 1989;140(2):531-554. 17. Arthur JR. The glutathione peroxidases. Cell Mol Life Sci. 2000;57(13-14):1825-1835. 18. Pincemail J, Kolh P, Detry O, et al. Antioxidant status after cold ischemia of rabbit lung. Transplant Proc. 2000;32(2):484-485. 19. Szasz T, Thakali K, Fink GD, Watts SW. A comparison of arteries and veins in oxidative stress: producers, destroyers, function, and disease. Exp Biol Med (Maywood).;232(1):27-37. 20. Kinnula VL, Crapo JD, Raivio KO. Generation and disposal of reactive oxygen metabolites in the lung. Lab Invest. 1995;73(1):3- 19. 21. Kinnula VL, Pääkkö P, Soini Y. Antioxidant enzymes and redox regulating thiol proteins in malignancies of human lung. FEBS Lett. 2004;569(1-3):1-6. 22. Al-Mehdi AB, Song C, Tozawa K, Fisher AB. Ca2+- and phosphatidylinositol 3-kinase-dependent nitric oxide generation in lung endothelial cells in situ with ischemia. J Biol Chem. 2000;275(51):39807-39810.    less

Abstract : Objectives: Our purpose was to investigate antioxidant enzymes and lipid peroxidation in time cou    more

Abstract : Objectives: Our purpose was to investigate antioxidant enzymes and lipid peroxidation in time course ischemic lung preservation in rats. Materials and Methods: Thirty-six Wistar rats were divided into 6 groups of 6 rats each. After having been anesthetized, the rats were intubated and connected to a rodent ventilator. Lung-heart blocks were excised. In the control group, the lungs were immediately stored at –80°C after removal. The lungs from the other groups were preserved in 40 mililiters of low potassium dextran solution at 4°C for 6, 12, 24, 48, and 72 hours, respectively. Antioxidant enzyme activity and malondialdehyde levels were then measured. Results: Superoxide dismutase activity significantly increased at the 12th hour and remained higher up to the 72nd hour (P < .001). Glutathione peroxidase activity was higher than that in the control group from the 6th to the 24th hour but was significant only at the 12th hour (P < .001) and decreased below the level in the control group after the 48th hour. Catalase activity was significantly higher than that in the control group in all preservation periods (P < .001). The nitric oxide level slowly increased and reached a significantly higher level than that in the control group at the 24th and 72nd hours (P = .028) and then decreased to the level found in the control group. The malondialdehyde level slightly increased from the 6th to the 24th hour, but that increase, when compared with the level in the control group, had no statistical significance (P = .110). Conclusions: In ischemic lung preservation, oxidative stress begins during the early phase of preservation and continues for up to 72 hours. Although oxidative stress continues for a significant period, an antioxidant mechanism adequately prevents its harmful effects on the lung. Thus no significant lipid peroxidation occurred in long-term ischemic lung preservation in the murine model studied.- Slides    less