Abstract : Arsenic is a metalloid that causes the dysfunction of critical enzymes oxidative stress and maligna
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Abstract : Arsenic is a metalloid that causes the dysfunction of critical enzymes oxidative stress and malignancies. In recent years several transporters of As(III) have been identified including aquaglyceroporins (AQP) and multidrug resistance proteins (MRP). As(V) transport however has not been sufficiently studied because it has been assumed that arsenate is taken up by mammalian cells through inorganic phosphate (Pi) transporters. In this paper we have analyzed the role of Pi transporters in the uptake of arsenate by directly using (73)As(V) as a radiotracer in phosphate transporter-expressing Xenopus laevis oocytes. The affinities of Pi transporters for H(3)AsO(4) were lower than the affinities for Pi. NaPiIIa NaPiIIc Pit1 and Pit2 showed a K(m) for arsenate that was gt1mM (i.e. at least ten times lower than the affinities for Pi). The NaPiIIb isoform showed the highest affinity for As(V) in mouse (57 microM) rat (51 microM) and human (9.7 microM) which are very similar to the affinities for Pi. Therefore NaPiIIb can have a prominent role in the toxicokinetics of arsenic following oral exposure to freshwater or food contaminated with As(V).(c) 2010 Elsevier Inc. All rights reserved.PMID 20510259 PubMed - indexed for MEDLINEPublication Types MeSH Terms SubstancesPublication Types Research Support Non-U.S. GovtMeSH TermsAnimalsArsenates/metabolism*Arsenates/toxicityBiological Transport ActiveHumansMiceOocytes/metabolismRatsSodium-Phosphate Cotransporter Proteins Type IIb/metabolism*Xenopus laevisSubstancesArsenatesSodium-Phosphate Cotransporter Proteins Type IIbarsenic acidLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information ServicesOther Literature SourcesCOS Scholar UniverseMolecular Biology DatabasesARSENIC COMPOUNDS - HSDBARSENIC ACID - HSDB Supplemental Content Related citations Role of rat sodium/phosphate cotransporters in the cell membrane transport of arsenate. Toxicol Appl Pharmacol. 2008 Role of rat sodium/phosphate cotransporters in the cell membrane transport of arsenate.Villa-Bellosta R Sorribas V. Toxicol Appl Pharmacol. 2008 Oct 1 232(1)125-34. Epub 2008 Jun 10. Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes. J Physiol. 1999 Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes.Forster IC Traebert M Jankowski M Stange G Biber J Murer H. J Physiol. 1999 Jun 1 517 ( Pt 2)327-40. Characterization of transport mechanisms and determinants critical for Na+-dependent Pi symport of the PiT family paralogs human PiT1 and PiT2. Am J Physiol Cell Physiol. 2006 Characterization of transport mechanisms and determinants critical for Na+-dependent Pi symport of the PiT family paralogs human PiT1 and PiT2.Bøttger P Hede SE Grunnet M Høyer B Klaerke DA Pedersen L. Am J Physiol Cell Physiol. 2006 Dec 291(6)C1377-87. Epub 2006 Jun 21. Review Odontoblast phosphate and calcium transport in dentinogenesis. Swed Dent J Suppl. 2002 Review Odontoblast phosphate and calcium transport in dentinogenesis.Lundquist P. Swed Dent J Suppl. 2002 (154)1-52. Review Molecular characteristics of mammalian and insect amino acid transporters implications for amino acid homeostasis. J Exp Biol. 1997 Review Molecular characteristics of mammalian and insect amino acid transporters implications for amino acid homeostasis.Castagna M Shayakul C Trotti D Sacchi VF Harvey WR Hediger MA. J Exp Biol. 1997 Jan 200(Pt 2)269-86. See reviews... See all... All links from this record Related Citations Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant with the “linked from” citation displayed first. Compound (MeSH Keyword) PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles. Substance (MeSH Keyword) PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles. Recent activity Clear Turn Off Turn On Arsenate transport by sodium/phosphate cotransporter type IIb. Arsenate transport by sodium/phosphate cotransporter type IIb.Toxicol Appl Pharmacol. 2010 Aug 15 247(1)36-40. Epub 2010 May 25 . PubMed Your browsing activity is empty. Activity recording is turned off. Turn recording back on See more... 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