Abstract : The aim of this study was to characterise the molecular epidemiology and mechanisms of carbapenem r
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Abstract : The aim of this study was to characterise the molecular epidemiology and mechanisms of carbapenem resistance of nosocomial Acinetobacter baumannii isolates in a new university hospital in Turkey. A total of 145 carbapenem-resistant A. baumannii (CRAB) isolates were collected during the period 2003-2006. All isolates were typed by amplified fragment length polymorphism (AFLP) analysis. AFLP analysis showed three predominant clusters consisting of 72 20 and 12 clinical strains as well as some smaller clusters and 23 unique strains. The three main clonal AFLP types corresponded to three major antibiotic susceptibility patterns. One environmental isolate was found related to the major outbreak clone. The reference type strains of European clones I II and III were also typed by AFLP and analysed for clonal similarity. Polymerase chain reaction (PCR) analysis of different carbapenem resistance genes showed that strains from each of the three main clusters as well as 79 of the remaining strains harboured the bla(OXA-58) gene. No genes encoding the metallo-beta-lactamases GIM-1 SIM-1 SPM-1 IMP-like and VIM-like or the oxacillinases OXA-24-like and OXA-23-like were detected. In conclusion multiple clones of CRAB strains producing OXA-58-type oxacillinase were responsible for a sustained CRAB outbreak occurring in a hospital in Turkey. These isolates were not associated with A. baumannii strains of the major European clones I II or III.Copyright (c) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.PMID 20510587 PubMed - indexed for MEDLINEMeSH Terms SubstancesMeSH TermsAcinetobacter Infections/epidemiology*Acinetobacter Infections/microbiologyAcinetobacter baumannii/drug effectsAcinetobacter baumannii/enzymologyAcinetobacter baumannii/genetics*AdolescentAdultAgedAged 80 and overAnti-Bacterial Agents/pharmacology*Carbapenems/pharmacology*ChildDisease Outbreaks*Drug Resistance Bacterial/geneticsFemaleGenes Bacterial/geneticsHospitals UniversityHumansMaleMicrobial Sensitivity TestsMiddle AgedMolecular EpidemiologyTurkey/epidemiologybeta-Lactamases/metabolismSubstancesAnti-Bacterial AgentsCarbapenemsbeta-Lactamasesbeta-lactamase OXA-58 Acinetobacter baumanniiLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information ServicesMedicalAntibiotics - MedlinePlus Health Information Supplemental Content Related citations Increasing carbapenem resistance due to the clonal dissemination of oxacillinase (OXA-23 and OXA-58)-producing Acinetobacter baumannii report from the Turkish SENTRY Program sites. J Med Microbiol. 2008 Increasing carbapenem resistance due to the clonal dissemination of oxacillinase (OXA-23 and OXA-58)-producing Acinetobacter baumannii report from the Turkish SENTRY Program sites.Gur D Korten V Unal S Deshpande LM Castanheira M. J Med Microbiol. 2008 Dec 57(Pt 12)1529-32. Clusters of imipenem-resistant Acinetobacter baumannii clones producing different carbapenemases in an intensive care unit. Clin Microbiol Infect. 2008 Clusters of imipenem-resistant Acinetobacter baumannii clones producing different carbapenemases in an intensive care unit.Tsakris A Ikonomidis A Poulou A Spanakis N Vrizas D Diomidous M Pournaras S Markou F. Clin Microbiol Infect. 2008 Jun 14(6)588-94. Epub 2008 Apr 5. Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II. J Antimicrob Chemother. 2008 Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II.Nemec A Krízová L Maixnerová M Diancourt L van der Reijden TJ Brisse S van den Broek P Dijkshoorn L. J Antimicrob Chemother. 2008 Sep 62(3)484-9. Epub 2008 May 13. A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58. J Antimicrob Chemother. 2005 A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58.Héritier C Dubouix A Poirel L Marty N Nordmann P. J Antimicrob Chemother. 2005 Jan 55(1)115-8. Epub 2004 Dec 8. Review Carbapenem resistance in Acinetobacter baumannii mechanisms and epidemiology. Clin Microbiol Infect. 2006 Review Carbapenem resistance in Acinetobacter baumannii mechanisms and epidemiology.Poirel L Nordmann P. Clin Microbiol Infect. 2006 Sep 12(9)826-36. See reviews... See all... All links from this record Related Citations Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant with the “linked from” citation displayed first. Substance (MeSH Keyword) PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles. Recent activity Clear Turn Off Turn On Characterisation of carbapenem-resistant Acinetobacter baumannii outbreak strain... Characterisation of carbapenem-resistant Acinetobacter baumannii outbreak strains producing OXA-58 in Turkey.Int J Antimicrob Agents. 2010 Aug 36(2)114-8. Epub 2010 May 26 . PubMed Your browsing activity is empty. Activity recording is turned off. Turn recording back on See more... 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