Abstract : This study was performed to evaluate the infectivity of bradyzoites of two Besnoitia caprae isolate
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Abstract : This study was performed to evaluate the infectivity of bradyzoites of two Besnoitia caprae isolates BC-1 and BC-2 to inbred BALB/c mice. Each group of inbred BALB/c mice was inoculated intraperitoneally with 1 x 10(3) 1 x 10(4) 1 x 10(5) 5 x 10(5) and 1 x 10(6) of one of the two isolates of B. caprae bradyzoites. The mice were monitored daily for a period of 40 days for survival. After death of each mice several passages from its peritoneal washing and tissues were analyzed using ribosomal DNA-specific PCR assay. Marked differences in pathogenicity between the isolates were seen. All the inbred BALB/c mice infected with BC-2 survived but all the mice that were administered with 1 x 0(5) 5 x 10(5) and 1 x 10(6) BC-1 bradyzoites were died within 4-9 days post-infection (DPI). Histopathological examination of the tissues of the dead mice revealed hyperemia and necrosis with presence of mononuclear and polymorphonuclear cell infiltration in myocardium spleen and intestines together with interstitial pneumonia and peritonitis. All inbred BALB/c mice in the 1 x 10(3) and 1 x 10(4) groups of BC-1 inoculated mice survived and they were euthanized after 40 DPI. Chronic inflammation with infiltration of mononuclear cells was evident in myocardium spleen alveolar septa of the lungs of most of the examined tissues with hemorrhagic enteritis in the mice infected with 1 x 10(6) bradyzoites. The mice infected with different doses of BC-2 were euthanized after 40 DPI and no lesion was seen in histopathological sections of their organs. All peritoneal washings and examined tissues were PCR positive in BC-1 group. This experiment is the first report to show inbred BALB/c mice as a relevant model for B. caprae and demonstrates that this strain of inbred BALB/c mice is a suitable animal model for biological studies and examination of pathogenesis for this species of Besnoitia. The present findings also provide evidence for significant differences between the two isolates of B. caprae.
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