Abstract : PURPOSE The effect of cold low-pressure plasma treatment on neovascularization of a dermis substitu
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Abstract : PURPOSE The effect of cold low-pressure plasma treatment on neovascularization of a dermis substitute was evaluated in a mouse model.|MATERIAL AND METHODS Collagen-elastin matrices (Matriderm(®)) were used as scaffolds. Low-pressure argon/hydrogene plasma-treated scaffolds were transplanted into the dorsal skinfold chambers of balb/c mice (group 1 n=10). Untreated scaffolds served as controls (group 2 n=10). Intravital fluorescence microscopy was performed within the border zone of the scaffolds on days 1 5 and 10. Functional vessel density (FVD) vessel diameter intervascular distance microvascular permeability and leukocyte-endothelium interaction were analyzed.|RESULTS An increase of FVD associated with a reduction of the intervascular distance was observed. Statistical analysis revealed that the functional vessel density in the border zone of the scaffolds was significantly enhanced in the plasma-treated group compared to controls. For group 1 an increase of FVD from 282±8 cm/cm(2) on days 5 to 315±8 cm/cm(2) on day 10 was observed. Whereas values of 254±7 cm/cm(2) on day 5 and 275±13 cm/cm(2) on day 10 have resulted in group 2 (mean±S.E.M. Students t-test plt0.05).|CONCLUSION: The surface treatment by cold low-pressure plasma intensifies the angiogenesis and accelerates the neovascularization of collagen-elastin matrix.|
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