Abstract : The objective of this study was to assess whether it is possible to minimise the variance in colony
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Abstract : The objective of this study was to assess whether it is possible to minimise the variance in colony counts on replicate target samples of foods by aseptic compositing of the samples or by increasing the quantity of sample examined. The results show that compositing reduces the overall variance and hence the standard deviation to very low levels although in some cases the overall variance remains relatively high reflecting the heterogeneous distribution of microorganisms in the foods. Increasing the weight of target sample examined (e.g. from 10 g to 100g) had a pronounced effect on the mean log(10) colony count and significantly reduced the variance of the mean. The results are discussed in relation to the quantity of sample that is recommended for examination in international and other standards.Copyright 2010 Elsevier Ltd. All rights reserved.PMID 20510777 PubMed - indexed for MEDLINEPublication Types MeSH TermsPublication Types Evaluation StudiesResearch Support Non-U.S. GovtMeSH TermsAnimalsBacteria/growth amp developmentBacteria/isolation amp purification*Bacteriological Techniques*CattleColony Count MicrobialMeat Products/microbiology*Milk/microbiology*SwineLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information ServicesOther Literature SourcesCOS Scholar Universe Supplemental Content Related citations Evaluation of a conductance method for enumerating Escherichia coli on chicken pork fish beef and milk. J Food Prot. 1999 Evaluation of a conductance method for enumerating Escherichia coli on chicken pork fish beef and milk.Edmiston AL Russell SM. J Food Prot. 1999 Nov 62(11)1260-5. Substrate supporting disc method for confirmed detection of total coliforms and E. coli in all foods collaborative study. J AOAC Int. 1993 Substrate supporting disc method for confirmed detection of total coliforms and E. coli in all foods collaborative study.Feldsine PT Falbo-Nelson MT Hustead DL. J AOAC Int. 1993 Sep-Oct 76(5)988-1005. Bacterial profile of ground beef made from carcass tissue experimentally contaminated with pathogenic and spoilage bacteria before being washed with hot water alkaline solution or organic acid and then stored at 4 or 12 degrees C. J Food Prot. 1998 Bacterial profile of ground beef made from carcass tissue experimentally contaminated with pathogenic and spoilage bacteria before being washed with hot water alkaline solution or organic acid and then stored at 4 or 12 degrees C.Dorsa WJ Cutter CN Siragusa GR. J Food Prot. 1998 Sep 61(9)1109-18. Review Comparison of direct colony count methods and the MPN-method for quantitative detection of Listeria in model and field conditions. Berl Munch Tierarztl Wochenschr. 2001 Review Comparison of direct colony count methods and the MPN-method for quantitative detection of Listeria in model and field conditions.Hildebrandt G Schott W. Berl Munch Tierarztl Wochenschr. 2001 Nov-Dec 114(11-12)453-64. Review Lessons from the organization of a proficiency testing program in food microbiology by interlaboratory comparison analytical methods in use impact of methods on bacterial counts and measurement uncertainty of bacterial counts. Food Microbiol. 2006 Review Lessons from the organization of a proficiency testing program in food microbiology by interlaboratory comparison analytical methods in use impact of methods on bacterial counts and measurement uncertainty of bacterial counts.Augustin JC Carlier V. Food Microbiol. 2006 Feb 23(1)1-38. See reviews... See all... Recent activity Clear Turn Off Turn On Minimising the between-sample variance in colony counts on foods. Minimising the between-sample variance in colony counts on foods.Food Microbiol. 2010 Aug 27(5)598-603. Epub 2010 Feb 16 . PubMed Your browsing activity is empty. Activity recording is turned off. Turn recording back on See more... You are here NCBI gt Literature gt PubMed Write to the Help Desk Simple NCBI Directory Getting Started NCBI Education NCBI Help Manual NCBI Handbook Training amp Tutorials Resources Chemicals amp BioassaysData amp SoftwareDNA amp RNADomains amp StructuresGenes amp ExpressionGenetics amp MedicineGenomes amp MapsHomologyLiteratureProteinsSequence AnalysisTaxonomyTraining amp TutorialsVariation Popular PubMed Nucleotide BLAST PubMed Central Gene Bookshelf Protein OMIM Genome SNP Structure Featured GenBank Reference Sequences Map Viewer Genome Projects Human Genome Mouse Genome Influenza Virus Primer-BLAST Sequence Read Archive NCBI Information About NCBI Research at NCBI NCBI Newsletter NCBI FTP Site NCBI on Facebook NCBI on Twitter NCBI on YouTube NIH DHHS USA.gov Copyright | Disclaimer | Privacy | Accessibility | Contact National Center for Biotechnology Information U.S. National Library of Medicine 8600 Rockville Pike Bethesda MD 20894 USA
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