Abstract : The epidermal growth factor receptor (EGFR) is a key driver in the process of squamous cell carcino
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Abstract : The epidermal growth factor receptor (EGFR) is a key driver in the process of squamous cell carcinoma (SCC) cell mitogenesis. Phospholipase C-gamma1 (PLC-gamma1) is a downstream target of EGFR signaling but the role and necessity of PLC-gamma1 in EGFR-induced cell mitogenesis remain unclear. In the present study we report an elevated expression of PLC-gamma1 in human SCC biopsies relative to adjacent normal epidermis and in human SCC cell lines compared to normal human keratinocytes. EGFR-induced SCC cell mitogenesis was blocked by small interfering RNA knockdown of PLC-gamma1. However inhibition of the catalytic activity of phospholipase C had no effect on EGFR-induced SCC cell mitogenesis. In response to the EGFR ligand epidermal growth factor (EGF) PLC-gamma1 was translocated not only to the plasma membrane but also to the nucleus. These data suggest that PLC-gamma1 is required for EGFR-induced SCC cell mitogenesis and the mitogenic function of PLC-gamma1 is independent of its lipase activity.Copyright (c) 2010 Elsevier Inc. All rights reserved.PMID 20510673 PubMed - indexed for MEDLINEPMCID PMC2901589 Available on 2011/6/1Publication Types MeSH Terms Substances Grant SupportPublication Types Research Support N.I.H. ExtramuralMeSH TermsCarcinoma Squamous Cell/enzymologyCarcinoma Squamous Cell/pathology*Cell Line TumorCell Membrane/enzymologyCell NucleusEpidermal Growth Factor/pharmacologyGene Knockdown TechniquesHumansKeratinocytes/enzymologyMitosis*Phospholipase C gamma/geneticsPhospholipase C gamma/metabolism*Protein TransportRNA Small Interfering/geneticsReceptor Epidermal Growth Factor/metabolism*SubstancesRNA Small InterferingEpidermal Growth FactorReceptor Epidermal Growth FactorPhospholipase C gammaGrant Support1R03DE018001/DE/NIDCR NIH HHS/United States1R21DE019529-01A2/DE/NIDCR NIH HHS/United StatesLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOSwets Information Services Supplemental Content Related citations The SH3 domain but not the catalytic domain is required for phospholipase C-gamma1 to mediate epidermal growth factor-induced mitogenesis. Biochem Biophys Res Commun. 2010 The SH3 domain but not the catalytic domain is required for phospholipase C-gamma1 to mediate epidermal growth factor-induced mitogenesis.Xie Z Chen Y Pennypacker SD Zhou Z Peng D. Biochem Biophys Res Commun. 2010 Aug 6 398(4)719-22. Epub 2010 Jul 16. Critical role for the catalytic activity of phospholipase C-gamma1 in epidermal growth factor-induced cell migration. Biochem Biophys Res Commun. 2010 Critical role for the catalytic activity of phospholipase C-gamma1 in epidermal growth factor-induced cell migration.Xie Z Peng J Pennypacker SD Chen Y. Biochem Biophys Res Commun. 2010 Aug 27 399(3)425-8. Epub 2010 Jul 30. Requirement for phospholipase C-gamma1 enzymatic activity in growth factor-induced mitogenesis. Mol Cell Biol. 1998 Requirement for phospholipase C-gamma1 enzymatic activity in growth factor-induced mitogenesis.Wang Z Glück S Zhang L Moran MF. Mol Cell Biol. 1998 Jan 18(1)590-7. Effect of epidermal growth factor receptor internalization on regulation of the phospholipase C-gamma1 signaling pathway. J Biol Chem. 1999 Effect of epidermal growth factor receptor internalization on regulation of the phospholipase C-gamma1 signaling pathway.Haugh JM Schooler K Wells A Wiley HS Lauffenburger DA. J Biol Chem. 1999 Mar 26 274(13)8958-65. Review Interactions between the epidermal growth factor receptor and type I protein kinase A biological significance and therapeutic implications. Clin Cancer Res. 1998 Review Interactions between the epidermal growth factor receptor and type I protein kinase A biological significance and therapeutic implications.Ciardiello F Tortora G. Clin Cancer Res. 1998 Apr 4(4)821-8. See reviews... See all... All links from this record Related Citations Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. 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UniGene UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records. GEO Profiles Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile. Recent activity Clear Turn Off Turn On Phospholipase C-gamma1 is required for the epidermal growth factor receptor-indu... Phospholipase C-gamma1 is required for the epidermal growth factor receptor-induced squamous cell carcinoma cell mitogenesis.Biochem Biophys Res Commun. 2010 Jun 25 397(2)296-300. Epub 2010 May 26 . PubMed Your browsing activity is empty. Activity recording is turned off. Turn recording back on See more... 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