Abstract : Reprogramming human somatic cells to pluripotency represents a valuable resource for research aimin
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Abstract : Reprogramming human somatic cells to pluripotency represents a valuable resource for research aiming at the development of in vitro models for human diseases and regenerative medicines to produce patient-specific induced pluripotent stem (iPS) cells. Seeking appropriate cell resources for higher efficiency and reducing the risk of viral transgene activation especially oncogene activation are of significance for iPS cell research. In this study we tested whether human amnion-derived cells (hADCs) could be rapidly and efficiently reprogrammed into iPS cells by the defined factors OCT4/SOX2/NANOG. hADCs from normal placenta were isolated and cultured. The 3rd passage cells were infected with the lentiviral vectors for the delivery of OCT4 SOX2 and NANOG. Afterwards the generated iPSCs were identified by morphology pluripotency markers global gene expression profiles and epigenetic status both in vitro and in vivo. The results showed that we were able to reprogram hADCs by the defined factors (OCT4/SOX2/NANOG). The efficiency was significantly high (about 0.1) and the typical colonies appeared on the 9th day after infection. They were similar to human embryonic stem (ES) cells in morphology proliferation surface markers gene expression and the epigenetic status of pluripotent cell-specific genes. Furthermore these cells were able to differentiate into various cell types of all three germ layers both in vitro and in vivo. These results demonstrate that hADCs were an ideal somatic cell resource for the rapid and efficient generation of iPS cells by OCT4/SOX2/NANOG.Copyright © 2010. Published by Elsevier B.V.PMID 20510497 PubMed - in processPublication TypesPublication Types Research Support Non-U.S. GovtLinkOut - more resourcesFull Text SourcesBlackwell PublishingElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information Services Supplemental Content Related citations Generation of human-induced pluripotent stem cells from gut mesentery-derived cells by ectopic expression of OCT4/SOX2/NANOG. Cell Reprogram. 2010 Generation of human-induced pluripotent stem cells from gut mesentery-derived cells by ectopic expression of OCT4/SOX2/NANOG.Li Y Zhao H Lan F Lee A Chen L Lin C Yao Y Li L. Cell Reprogram. 2010 Jun 12(3)237-47. Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells. Hum Mol Genet. 2009 Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells.Li C Zhou J Shi G Ma Y Yang Y Gu J Yu H Jin S Wei Z Chen F et al. Hum Mol Genet. 2009 Nov 15 18(22)4340-9. Epub 2009 Aug 13. 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Curr Stem Cell Res Ther. 2009 Sep 4(3)168-77. See reviews... See all... Recent activity Clear Turn Off Turn On Rapid and efficient reprogramming of human amnion-derived cells into pluripotenc... Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG.Differentiation. 2010 Sep-Oct 80(2-3)123-9. Epub 2010 May 26 . PubMed Your browsing activity is empty. Activity recording is turned off. Turn recording back on See more... 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