Abstract : This study supplied a simple extraction method for intact soybean oil body (ISOB) and examined the
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Abstract : This study supplied a simple extraction method for intact soybean oil body (ISOB) and examined the heating effect on ISOB. ISOB which just contained its intrinsic protein (oleosin) could be obtained by pH 11 extraction (50000g 45 min). ISOB suspension was dialyzed to deionized water (13600) and named DISOB. DISOB aggregated at pH 5.7 but NaCl pre-addition (5-500 mM) made ISOB disperse well at pH 5.7. The heating (30 40 50 60 70 80 and 90 degrees C and boiling water baths 30 min) did not affect the particle size distributions of ISOB. The pH and CaCl(2) effects on DISOB and its surface hydrophobicity were also not affected by heating (gt95 degrees C 5 min). Both unheated and heated ISOB were bound to native soybean protein but were not bound to the heat-denatured one. Thus it was suggested that ISOB changed little by heating. This study was meaningful in two aspects (1) pH 11 extraction removed beta-conglycinin glycinin and allergenic proteins (such as Gly m Bd 30K) and the obtained ISOB had good stability in an aqueous medium. (2) Heating could denature the contamination allergenic proteins.
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